stable isotope labeling การใช้

• TAILS is also compatible with Stable isotope labeling by amino acids in cell culture ( SILAC ).
• The figure demonstrates the use of stable isotope labeling to discover the carbon atom rearrangement through reactions using position specific labeled compounds.
• Metabolic flux analysis ( MFA ) using stable isotope labeling is an important tool for elucidating the flux of certain elements through the metabolic pathways and products, through each reaction.
• Stable isotope labeling by amino acids in cell culture ( SILAC ) has emerged as an important method in secretomics  it helps to distinguish between secreted proteins and bovine serum contaminants in cell culture.
• One important application is in " Stable isotope labeling with amino acids in cell culture " ( SILAC ) . 13 C-enriched compounds are used in medical diagnostic tests such as the urea breath test.
• "' SILAC "'( "'Stable Isotope Labeling by / with Amino acids in Cell culture "') is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling.
• In proteomics, the study of the full set of proteins expressed by a genome, identifying diseases biomarkers can involve the usage of stable isotope labeling by amino acids in cell culture ( SILAC ), that provides isotopic labeled forms of amino acid used to estimate protein levels.
• Relative quantification methods include isotope-coded affinity tags ( ICAT ), isobaric labeling ( tandem mass tags ( TMT ) and isobaric tags for relative and absolute quantification ( iTRAQ ) ), label-free quantification Metal-coded tags ( MeCAT ), N-terminal labelling, stable isotope labeling with amino acids in cell culture ( SILAC ), and Terminal amine isotopic labeling of substrates ( TAILS ).